Sanger Sequencing

Welcome to the Roswell Park Cancer Institute Sanger Sequencing Laboratory homepage! Hopefully, this website will provide you with the whos, whats, wheres, whens and whys of DNA sequencing at the RPCI Sanger Sequencing lab and enable you to get good sequence data, quickly and efficiently, for your DNA samples. Click on the links to the left to direct you to a specific area of interest or just to browse.

A quick glimpse of the information available at these links is outlined below to help you get started.

Overview of the Sanger Sequencing Laboratory - click here for general information about the sequencing lab, such as pricing, services we provide, where we are located, hours of operation, how to get your samples to us if you are off-campus and other details.

Online ordering -  With our web-based ordering system, you will be able to submit your orders electronically and retrieve your sequence data when completed. This online resource is now available at http://biopolymer.roswellpark.org. (In Internet Explorer, you must cut and paste the link.)

Sequencing basics - select this link for an overview of the basics of automated fluorescent sequencing and how it works. Also located in this section is a table of types of DNA we can sequence and required concentrations, our recommendations for template preparation, primer design, and other useful advice.

Interpreting your chromatograms - go to this section for some advice on how to evaluate your chromatogram and sequence data quality. See what's good and bad, and what can be expected for a typical DNA sequence.

Troubleshooting your data - so we tell you that your DNA sample failed to give any sequence data. Or perhaps it had weak signal. Or maybe your sample showed double peaks. Go to this section to read about some examples of common sequencing dilemmas, reasons why you might encounter them, and what you can do to try and solve these problems.

Primers we provide - our facility provides numerous primers, free of charge, to be used when sequencing many of the more common vectors. A table here lists their names, sequences and molecular weights.

ABI viewers - in order to view the ABI color files we now provide for downloading from our server, you will need specific software to open and work with these files. In this section, we provide links to websites offering freeware for downloading to your computer.

Useful links - here is a list of some useful websites we've come across or heard about over the years. If you have a favorite that you think others might find useful, let us know and we'll add it!

 

The DNA sequencing services provided by RPCI’s Genomic Shared Resource are intended solely for research purposes.  These DNA sequencing services are not intended or certified by the New York State Department of Health for use in clinical diagnostic testing or treatment, including but not limited to patient education.  
 
Roswell Park Cancer Institute shall have no liability for claims by, or damages of any kind whatsoever to, a user of these DNA sequencing services for a decision or action taken in reliance on the information provided in the DNA sequencing.  Such damages include, without limitation, direct, indirect, special, incidental or consequential damages. 

For questions or problems with this website, contact michael.logar@roswellpark.org.